Identification of Balamuthia mandrillaris by PCR assay using the mitochondrial 16S rRNA gene as a target.
نویسندگان
چکیده
Balamuthia mandrillaris is an opportunistic pathogen that causes granulomatous amebic meningoencephalitis in animals, including humans. Based on sequence analysis of mitochondrial small-subunit-rRNA genes, we developed primers that amplify a Balamuthia-specific PCR product. These primers will be useful for retrospective analyses of fixed tissues and possible identification of Balamuthia in vivo.
منابع مشابه
Detection of Balamuthia mitochondrial 16S rRNA gene DNA in clinical specimens by PCR.
Balamuthia mandrillaris is a free-living ameba that causes granulomatous amebic encephalitis in both immunocompromised and immunocompetent individuals. Because of a lack of pathognomonic symptoms and the difficulty in recognizing amebas in biopsied tissues, most cases are not diagnosed or effectively treated, leading to a >95% mortality. We report here on five cases of balamuthiasis that were d...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 41 1 شماره
صفحات -
تاریخ انتشار 2003